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Intracellular Fate of Sub-Toxic Concentration of Functionalized Selenium Nanoparticles in Aggressive Prostate Cancer Cells
,
Olivier Proux ,
Salvatore Gazze,
Odile Filhol ,
BENOIT TOUBHANS,
Lucie Sauzéat ,
Sylvain Bouchet,
Aled Lewis,
Thierry Maffeis ,
Jean-Louis Hazemann ,
Sam Bayat ,
Peter Cloetens,
Steve Conlan ,
Laurent Charlet,
Sylvain Bohic
Nanomaterials, Volume: 13, Issue: 23, Start page: 2999
Swansea University Authors:
Salvatore Gazze, BENOIT TOUBHANS, Aled Lewis, Thierry Maffeis , Steve Conlan
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DOI (Published version): 10.3390/nano13232999
Abstract
Selenium 0 (Se0) is a powerful anti-proliferative agent in cancer research. We investigated the impact of sub-toxic concentrations of Se0 functionalized nanoparticles (SeNPs) on prostate cancer PC-3 cells and determined their intracellular localization and fate. An in-depth characterization of funct...
| Published in: | Nanomaterials |
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| ISSN: | 2079-4991 |
| Published: |
MDPI AG
2023
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa65073 |
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| Abstract: |
Selenium 0 (Se0) is a powerful anti-proliferative agent in cancer research. We investigated the impact of sub-toxic concentrations of Se0 functionalized nanoparticles (SeNPs) on prostate cancer PC-3 cells and determined their intracellular localization and fate. An in-depth characterization of functionalized selenium nanoparticles composition is proposed to certify that no chemical bias relative to synthesis issues might have impacted the study. Selenium is an extremely diluted element in the biological environment and therefore requires high-performance techniques with a very low detection limit and high spatial resolution for intracellular imaging. This was explored with state-of-the-art techniques, but also with cryopreparation to preserve the chemical and structural integrity of the cells for spatially resolved and speciation techniques. Monodisperse solutions of SeNPs capped with bovine serum albumin (BSA) were shown to slow down the migration capacity of aggressive prostate cancer cells compared to polydisperse solutions of SeNPs capped with chitosan. BSA coating could prevent interactions between the reactive surface of the nanoparticles and the plasma membrane, mitigating the generation of reactive oxygen species. The intracellular localization showed interaction with mitochondria and also a localization in the lysosome-related organelle. The SeNPs-BSA localization in mitochondria constitute a possible explanation for our result showing a very significant dampening of the PC-3 cell proliferation capabilities. The purpose of the use of sublethal compound concentrations was to limit adverse effects resulting from high cell death to best evaluate some cellular changes and the fate of these SeNPs on PC-3. Our findings provide new insight to further study the various mechanisms of cytotoxicity of SeNPs. |
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| Keywords: |
Prostate cancer, selenium, nanoparticle-correlative synchrotron imaging, X-ray fluorescence, speciation, cytotoxicity |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This research and C.B. were funded by the INCA (grant INCa-INSERM- ASC16019CSA “SEDMAC”). |
| Issue: |
23 |
| Start Page: |
2999 |